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小型蛋白垂直電泳槽

伯樂Mini-Protean Tetra蛋白電泳槽

伯樂Mini-Protean Tetra小型蛋白電泳槽可容納1~4塊小型手灌膠或Ready Gel 預(yù)制膠工作,用于蛋白SDS-PAGE垂直電泳分離。本欄目提供Mini-Protean Tetra的組成及選型配置方案。

設(shè)備名稱:蛋白垂直電泳槽  蛋白垂直電泳儀
制 造 商:美國Bio-Rad公司           產(chǎn) 地:新加坡/中國
品    牌:伯樂(Bio-Rad)    
型    號:Mini-Protean Tetra
電泳配件:見[主要附件]  商品價格:見[訂貨指南]

主要功能用途

Bio-Rad Mini- Protean Tetra蛋白垂直電泳槽,是Western Blot實(shí)驗(yàn)流程重要組成部件,可容納1~4 塊小型手灌膠或Ready Gel 預(yù)制膠(10×8.3cm)的電泳,主要用于蛋白聚丙烯酰胺凝膠電泳(PAGE或SDS-PAGE)實(shí)驗(yàn)。

 

性能特點(diǎn)

伯樂Mini-Protean Tetra蛋白垂直電泳槽,兼容Mini-Protean precast gels預(yù)制凝膠、Ready Gel precast gels預(yù)制凝膠或手工自制PAGE凝膠。

可在一小時內(nèi)完成多達(dá)四塊小型蛋白凝膠的電泳分離。

軟橡膠墊片與玻璃板形成嚴(yán)密密封,確保無泄漏灌膠。

帶有簡單凸輪的灌制框,確保在任何平面上實(shí)現(xiàn)精確對齊。

平行排列的灌膠架,能同時灌制兩塊凝膠,彈簧杠桿設(shè)計使得軟橡膠沉淀產(chǎn)生良好的密封性。

封邊墊條的長玻板加厚,不易破碎。

標(biāo)有厚度和孔數(shù)的玻板和加樣梳便于識別。

上樣向?qū)Х乐褂镜肋z漏上樣或重復(fù)上樣。

可互換模塊可將Mini- Protean Tetra電泳槽轉(zhuǎn)換為可進(jìn)行Western轉(zhuǎn)印的 Mini Trans-Blot轉(zhuǎn)印槽。

 

 

主要技術(shù)規(guī)格

伯樂Mini-Protean Tetra蛋白垂直電泳槽適用的凝膠類型包括:Mini- Protean預(yù)制膠、手灌膠

凝膠數(shù):1–4塊

凝膠格式:小型

適用的凝膠尺寸(寬×長)

預(yù)制膠:8.6×6.8cm;

手灌膠:8.3×7.3cm

玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm

緩沖液運(yùn)行需求

運(yùn)行2塊凝膠緩沖液總體積:700mL

運(yùn)行4塊凝膠緩沖液總體積:1000 mL

SDS-PAGE 典型運(yùn)行時間:35–45 分鐘(在200V恒定電壓下)

Mini-PROTEAN Tetra加樣梳每孔最大上樣量

1.0mm 10孔梳:44 μL

1.0mm 15孔梳:26 μL

 

安裝運(yùn)行條件

相對濕度范圍:0~95% R.H.

工作環(huán)境溫度:0℃~40℃

外觀尺寸(寬×長×高):12×16×18cm

Mini- Protean Tetra 電泳槽重量:1.0kg

(電泳電源另配)

 

組成配置

電泳槽系統(tǒng)

伯樂Mini-Protean Tetra蛋白電泳槽全套工作裝置包括電泳緩沖液槽、電極組件制膠組件輔助工具耗材。
按一次可運(yùn)行的最大凝膠數(shù)量,分為4-gel、2-gel兩種規(guī)格。
如需跑4塊凝膠,可訂購4凝膠槽,也可在2凝膠套裝基礎(chǔ)上加配Mini-Protean Tetra相應(yīng)功能附件,擴(kuò)展為4塊凝膠工作通量。

此外,根據(jù)使用的凝膠來源類型,還可單獨(dú)配置伯樂預(yù)制膠專用套裝。

品名

品牌

貨號

規(guī)格

描述

報價

蛋白垂直電泳槽

(4-gel/10-well/1.0mm)

Bio-Rad

1658001

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.0mm gel thickness

includes:

Tank, lid with power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.0mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽

(4-gel/10-well/1.5mm)

Bio-Rad

1658006

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.5mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.5mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽

(4-gel/10-well/0.75mm)

Bio-Rad

1658000

1套

Mini-PROTEAN Tetra Cell

4-gel system, 0.75mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 0.75mm combs

5×gel releasers(1653320)

詢價

詢價

蛋白垂直電泳槽l

(2-gel/10-well/1.0mm)

Bio-Rad

1658003

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.0mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 short plates and 5 spacer plates

詢價

詢價

蛋白垂直電泳槽

(2-gel/10-well/1.5mm)

Bio-Rad

1658007

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.5mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 sets of glass plates

詢價

詢價

蛋白垂直電泳槽

(2-gel/10-well/0.75mm)

Bio-Rad

1658002

1套

Mini-PROTEAN Tetra Cell

2-gel system, 0.75 mm gel thickness

includes:

1×casting stand, 2×casting   frames;

5×10-well combs;

5 short plates and 5 spacer plates

詢價

詢價

Mini Precast Gels(4-gel)

預(yù)制膠蛋白電泳槽

Bio-Rad

1658004

1套

Mini-PROTEAN Tetra Vertical Electrophoresis Cell

for Mini Precast Gels, 4-gel

includes:

electrode assembly;

companion running module;

tank, lid with power cables, cell buffer   dam

詢價

詢價

Mini Precast Gels (2-gel)

預(yù)制膠蛋白電泳槽

Bio-Rad

1658005

1套

Mini-PROTEAN Tetra Vertical Electrophoresis  Cell

for Mini Precast Gels, 2-gel

includes:

electrode assembly;

tank, lid with power cables, cell buffer dam

詢價

詢價



電泳槽組件

伯樂Mini-Protean Tetra 小型蛋白垂直電泳槽的制膠模塊(Casting Module),是指用于灌制特定厚薄和齒孔數(shù)目蛋白凝膠所需的模塊化套裝。

模塊由通用核心組件(Core System)和差異化功能組件組成。通用核心灌膠組件為灌膠架(含4個密封墊),夾膠框(casting frames)和或膠鏟(gel releaser)。差異化功能組件則包括特定規(guī)格的玻璃板套件,電泳梳和上樣導(dǎo)槽。

伯樂Mini-PROTEAN Tetra Cell小型垂直電泳槽有多種灌膠模塊可選,分別滿足不同上樣量和對凝膠厚度的特殊要求。

品名

品牌

貨號

規(guī)格

描述

報價

1.0mm/10孔 制膠模塊

Bio-Rad

1658015

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well/1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.0mm/15孔制膠模塊

Bio-Rad

1658016

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.0mm/5孔 制膠模塊

Bio-Rad

1658013

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates.

詢價

詢價

1.0mm/9孔 制膠模塊

Bio-Rad

1658014

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide.

詢價

詢價

1.5mm/10孔 制膠模塊

Bio-Rad

1658021

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well, 1.5 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.5mm/15孔 制膠模塊

Bio-Rad

1658022

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.5 mm gel casting module

includes:

2×casting stands, 4×casting frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

1.5mm/5孔 制膠模塊

Bio-Rad

1658019

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.5 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates.

詢價

詢價

1.5mm/9孔 制膠模塊

Bio-Rad

1658020

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.5 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/10孔 制膠模塊

Bio-Rad

1658010

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well,   0.75 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates

sample   loading guide.

詢價

詢價

0.75mm/15孔 制膠模塊

Bio-Rad

1658011

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/5孔 制膠模塊

Bio-Rad

1658008

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

0.75mm/9孔 制膠模塊

Bio-Rad

1658009

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

詢價

詢價

灌膠通用套件(4膠)

Bio-Rad

1658051

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

2×stands, 4×frames,4×gaskets;

5×gel   releasers.

適用于4膠Mini-Protean Tetra電泳槽

詢價

詢價

灌膠通用套件(2膠)

Bio-Rad

1658052

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

1×stand, 2×frames, 2×gaskets;

5×gel releasers.

適用于2膠Mini-Protean Tetra電泳槽

詢價

詢價

基本耗材

伯樂Mini-Protean Tetra垂直電泳槽工作的基本耗材主要包括蛋白凝膠玻璃板等。

品名

品牌

貨號

規(guī)格

描述

報價

1.0mm厚玻璃板

Bio-Rad

1653311

5塊/包

Spacer Plates with 1.0 mm Integrated Spacers

帶1.0mm厚封邊條,為1.0mm凝膠灌膠組件。

詢價

1.5mm厚玻璃板

Bio-Rad

1653312

5塊/包

spacer plate   with 1.5 mm integrated spacer

帶1.5mm厚封邊條,用于1.5mm凝膠的制備

詢價

薄玻璃板

Bio-Rad

1653308

5塊/包

short plate for Mini-PROTEAN Tetra System

 and Mini-PROTEAN 3 Multi-Casting Chamber.

外形尺寸(W×L):101× 73 mm

與帶任一厚度封邊墊條的長玻璃板配套使用

詢價

 

訂貨指南

1、蛋白電泳槽的選型

因不同實(shí)驗(yàn)樣品樣品數(shù)量、蛋白類型和表達(dá)豐度、實(shí)驗(yàn)經(jīng)驗(yàn)與操作習(xí)慣的不同,選擇蛋白電泳槽重點(diǎn)要結(jié)合所用凝膠的厚度(0.75mm、1.0mm、1.5mm)、每張凝膠電泳的上樣孔數(shù)(常用的10孔-最大上樣量66μl、15孔-40μl)、每輪電泳的凝膠通量(4膠、2膠)和凝膠類型(手灌膠、預(yù)制膠)來確定具體選型方案。

如單獨(dú)訂購Mini-Protean Tetra小型蛋白垂直電泳槽或更換電泳灌膠配件,請請參考本欄目“組成配置”“主機(jī)”部分所列各型電泳槽的技術(shù)規(guī)格和詳細(xì)配置內(nèi)容。最常用的1658001槽單元,其標(biāo)準(zhǔn)配置適用的是1.0mm/10-well/4-gel凝膠。

 

2、蛋白電泳槽電源的選擇

伯樂Mini-Protean Tetra小型蛋白垂直電泳槽本身工作運(yùn)行對電源并無特殊要求。伯樂PowerPac Basic 、PowerPac HC、PowerPac Universal等多個型號均可滿足同時給1-4個電泳槽供電的要求。

但須注意的是,通常情況下,蛋白電泳槽只是Western Blot實(shí)驗(yàn)流程的一個功能組件,單個電泳槽輸出凝膠數(shù)量(2塊或4塊)與電轉(zhuǎn)印槽(2塊)各自最大凝膠工作通量不同,要充分考慮實(shí)驗(yàn)流程上的有效銜接問題。

特別是當(dāng)實(shí)驗(yàn)樣品數(shù)量多于4塊凝膠的情況下,根據(jù)自身實(shí)驗(yàn)條件和經(jīng)驗(yàn),通盤考慮電轉(zhuǎn)印槽、電轉(zhuǎn)印模塊的配置數(shù)量。

與常規(guī)140V左右恒壓蛋白電泳跑膠不同,電轉(zhuǎn)印對電流要求較高。通常,每塊凝膠-印跡膜的工作電流可達(dá)200mA甚至更高。若同時進(jìn)行4塊凝膠轉(zhuǎn)膜操作,單一Basic電源很可能無法滿足供電要求。故需考慮HC、PowerPac Universal等具有電流輸出能力大于0.8A的電源型號確有必要。

 

3、電泳槽與與Mini-Trans Blot小型蛋白電泳轉(zhuǎn)印槽搭配

電轉(zhuǎn)移槽的選型配置問題,請參考Mini-Trans Blot小型蛋白轉(zhuǎn)印槽欄目內(nèi)容。


序號

品名

貨號

套裝配置描述

報價

1-1

Basic蛋白電泳套裝

(1.0mm/10-well/4-gel)

1658025

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac Basic power supply (1645050).

詢價

詢價

1-2

HC蛋白電泳套裝

(1.0mm/10-well/4-gel)

1658027

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac HC power supply (1645052).

詢價

詢價






2-1

電泳-轉(zhuǎn)印槽組合裝

(1.0mm/10-well/4-gel/2-blot)

1658029

Electrophoresis and blot Bundle (10-well/1.0mm/4-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658001);

Mini Trans-Blot Module (1703935).

詢價

詢價

2-2

Basic電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/2-blot)

1658033

Electrophoresis, blot and Power Supply   Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

2-3

HC電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/2-blot)

1658035

Electrophoresis, blot and Power Supply Bundle

for 10-well, 1.0 mm, 4-gel system

includes:

Mini-PROTEAN Tetra Cell (1658001);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052)

詢價

詢價

2-4

HC電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/4-gel/4-blot)

1658035-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Transfer Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

2-5

HC電源-電泳-轉(zhuǎn)印高級套裝

(1.0mm/10-well/4-gel)

(1.5mm/10-well/4-gel)

(4-blot)

1658035-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini-PROTEAN Tetra Cell Casting Module (1658021);

Mini Trans-Blot Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價






3-1

電泳-轉(zhuǎn)印槽組合裝

(1.5mm/10-well/2-gel/2-blot)

16580007-B

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN   Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935).

詢價

詢價

3-2

電泳-轉(zhuǎn)印組合裝

(1.5mm/10-well/4-gel/2-blot)

16580006-B

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

詢價

詢價

3-3

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/2-gel/2-blot)

16580007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

3-4

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/4-gel/2-blot)

16580006-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEA Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

3-5

HC電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/4-gel/4-blot)

16580006-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

Mini Trans-Blot Cell (1703930)

PowerPac HC Power Supply (1645052).

詢價

詢價






4-1

電泳-轉(zhuǎn)印槽組合裝

(1.0mm/10-well/2-gel)

1658003-C

Electrophoresis and blot Bundle (10-well/1.0mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658003);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-2

Basic電源-電泳-轉(zhuǎn)印套裝

(1.0mm/10-well/2-gel/2-blot)

1658003-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

4-3

電泳-轉(zhuǎn)印槽組合裝

(1.5mm/10-well/2-gel)

1658007-C

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658007);

Mini Trans-Blot Module (1703935).

詢價

詢價

4-4

Basic電源-電泳-轉(zhuǎn)印套裝

(1.5mm/10-well/2-gel/2-blot)

1658007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價






5-1

蛋白預(yù)制膠

電泳-轉(zhuǎn)印槽組合

1658030

Vertical Electrophoresis and Mini Trans-Blot Module Bundle

for Mini Precast 4-Gel

includes:

4-gel Mini-PROTEAN Vertical Cell (1658004);

Mini Trans-Blot Module (1703935).

詢價

詢價

5-2

蛋白預(yù)制膠

Basic電源電泳轉(zhuǎn)印套裝

1658034

Electrophoresis, blot and Power Supply Bundle

for precast 4-gel

includes:

Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

詢價

詢價

5-3

蛋白預(yù)制膠

HC電源電泳轉(zhuǎn)印套裝

1658036

Electrophoresis, blot and Power Supply Bundle

includes:

for Mini Precast Gels, 4-gel

Mini-PROTEAN Tetra Cell (1658004);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

詢價

詢價

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